HPLC Column, RP C18 ABX, 3um, 4.6mm ID x 100mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
The 85318‑10P Cogent RP C18 ABX™ HPLC Column is engineered for robust, high‑resolution separations under extreme mobile phase conditions, delivering consistent performance across a wide pH range (2–12).
Constructed with a bulky end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and improves peak shape for acidic, basic, and neutral analytes, particularly those with very high or very low pKa values.
✅ Ideal as a USP L1 alternate for methods involving strong acids or bases
100 mm Length – Increased Resolution for Analytical Methods
Higher resolving power than 50 mm columns
Improves separation of closely related compounds
Increases peak capacity for complex mixtures
Ideal for method development and refinement workflows
✅ Selected when greater resolution is required beyond fast screening methods
4.6 mm ID – Standard Analytical Platform
Compatible with standard HPLC systems
Ideal for:
routine QC analysis
analytical method development
Typical Flow Rate Range
~0.8–1.5 mL/min
✅ Provides reliable and reproducible analytical performance
3 µm Particle – Balanced Efficiency & Pressure
Higher efficiency than 5 µm columns
Produces:
Sharper peaks
Improved resolution
Lower backpressure than 1.8 µm UHPLC columns
✅ Ideal for:
High‑performance HPLC systems
Improving resolution without UHPLC requirements
Wide pH Stability (pH 2–12) – Extreme Method Capability
Designed for acidic and basic mobile phases
Suitable for:
Strong acids
Strong bases
Maintains integrity under aggressive analytical conditions
✅ Enables separations beyond standard C18 limitations
Why Choose RP C18 ABX™ Columns (3 µm)
Extreme pH Ruggedness
Stable across full pH range (2–12)
Ideal for challenging analytes and harsh methods
Balanced Analytical Performance
Combines efficiency and manageable backpressure
Enables use on standard HPLC platforms
Improved Peak Shape for Acids & Bases
Advanced end‑capping reduces secondary interactions
Enhances reproducibility and data quality
✅ Excellent for method development and analytical optimization
Application Performance Advantages
Suitable for:
Hydrophobic compounds
Neutral analytes
Long‑chain fatty acids
Enables:
Faster analysis with maintained resolution
Improved performance over legacy 5 µm methods
✅ Ideal for modernizing and optimizing existing analytical methods
System & Sample Considerations
Compatible with standard HPLC systems
Can be used on UHPLC systems if desired
Recommended sample preparation:
👉 Use 0.2 µm syringe filters for proper sample preparation before injection
✅ Helps:
Prevent column fouling
Maintain peak performance and lifetime
Helpful Resources & Links
Typical Applications
HPLC analysis
Acidic and basic compound separations
Pharmaceutical method development
Stability‑indicating methods
Long‑chain fatty acid analysis
QC and regulated analytical workflows
Important Usage Notes
Designed for standard analytical HPLC systems (4.6 mm ID)
Suitable for extreme pH mobile phases (pH 2–12)
Ideal for resolution‑focused method development
Monitor system pressure according to instrument limits